Journal: bioRxiv
Article Title: TOR mediates cytokinin-driven development and defense cues
doi: 10.1101/2022.03.07.483332
Figure Lengend Snippet: S. lycopersicum plants of the increased CK line pBLS>>IPT7 (“ IPT ”), the decreased CK line pFIL>>CKX3 (“ CKX ”), and their WT background M82, were treated with Mock (1:5000 DMSO in DDW) or 2 μM of the TOR inhibitors Torin2 ( A, B ) or WYE132 ( A ). Plants were challenged with B. cinerea (Bc) mycelia from a 72h old-culture, 24 h after drug treatment. A : Bc necrotic lesion size. B : percentage of disease reduction following TOR inhibition in the different genotypes. Bars represent mean ±SEM; boxplots represent inner quartile ranges (box), outer quartile ranges (whiskers), median (line in box), all points shown. Experiments were repeated 3 independent times. A : Asterisks indicate statistically significant disease reduction with Torin2 or WYE132 treatment, and different letters indicate statistically significant differences among samples, in a one-way ANOVA with a Tukey post hoc test, N=9, p<0.05 (****p<.0001, ***p<0.001, *p<0.05, ns-not significant). B: Different letters indicate statistically significant differences between samples in ANOVA with a Tukey post-hoc test, N=9, p<0.047.
Article Snippet: Torin2 (SML1224 Sigma-Aldrich) or WYE132 (PZ0321 Sigma-Aldrich), were applied to detached tomato leaves through the petiole for 24 hours prior to pathogen inoculation (both inhibitors), defense response quantification (only Torin2), or RNA preparation (only Torin2).
Techniques: Inhibition
